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利用含色氨酸的鉴定培养基从芝麻根际筛选出7株IAA产生菌,其中菌株SA4产IAA的能力最强。促生实验结果表明,菌株SA4明显具有促进黄瓜和玉米种子主根生长的能力,使它们的主根根长和根重增加为对照组的23倍。分子鉴定结果表明菌株SA4属于革兰氏阴性的假单胞菌属。
Abstract:Seven strains producing IAA were isolated from rhizosphere of sesame by the differential medium containing Ltryptophan,and strain SA4 was the best one producing IAA. The strain SA4 had obvious ability to promote the axial root growth of cucumber seedlings and maize seedlings. It made the axial root twice to three times as long as the control group,and also made the root fresh weight twice to three times as much as the control group. Strain SA4 was Gram negative one,and it was identified as Pseudomonas sp. based on the analyse of its 16 S r DNA.
[1]胡江春,薛德林,马成新,等.植物根际促生菌(PGPR)的研究与应用前景[J].应用生态学报,2004,15(10):1963-1966.
[2]Glick B R.The enhancement of plant growth by freeliving bacteria[J].Canadian Journal of Microbiology,1995,41(2):109-117.
[3]Arshad M,Frankenberger Jr W T.Microbial production of plant hormones[J].Plant and Soil,1991,133(1):1-8.
[4]Boddey R M,Dobereiner J.Nitrogen fixation associated with grasses and cereals:Recent progress and perspectives for the future[J].Nutrient Cycling in Agroecosystems,1995,42(1-3):241-250.
[5]Scher F M,Baker R.Effect of Pseudornonas putida and a synthetic iron chelator on induction of soil suppressiveness to Fusarium wilt pathogens[J].Phytopathology,1982,72:1567-1573.
[6]Shanahan P,O'Sullivan D J,Simpson P,et al.Isolation of 2,4-diacetylphloroglucinol from a fluorescent pseudomonad and investigation of physiological parameters influencing its production[J].Applied and Environmental Microbiology,1992,58(1):353-358.
[7]Flaishman M A,Eyal Z,Zilberstein A,et al.Suppression of Septoria tritici blotch and leaf rust of wheat by recombinant cyanide-producing strains of Pseudomonas putida[J].Molecular Plant-Microbe Interactions,1996,9(7):642-645.
[8]de Freitas J R,Banerjee M R,Germida J J.Phosphatesolubilizing rhizobacteria enhance the growth and yield but not phosphorus uptake of canola(Brassica napus L.)[J].Biology and Fertility of Soils,1997,24(4):358-364.
[9]Mantelin S,Touraine B.Plant growth-promoting bacteria and nitrate availability:impacts on root development and nitrate uptake[J].Journal of Experimental Botany,2004,55(394):27-34.
[10]Adesemoye A O,Torbert H A,Kloepper J W.Enhanced plant nutrient use efficiency with PGPR and AMF in an integrated nutrient management system[J].Journal of Microbiology(Canadian),2008,54(10):876-886.
[11]刘晓东,王补全,李伟.玉米根际促生菌的筛选及其对玉米生长发育的影响[J].安徽农业科学,2010,38(10):5043-5045.
[12]陈波,丁延芹,马海林,等.樱桃根际促生菌的筛选与鉴定[J].微生物学通报,2012,39(12):1746-1754.
[13]马娅莉,刘海燕.棉花根际促生菌的鉴定和促生效果[J].安徽农业科学,2012,40(32):15689-15691.
[14]刘秀花,梁峰.小麦根际促生菌的筛选与促生作用研究[J].安徽农业科学,2006,34(20):5300-5301,5308.
[15]李文英,彭智平,于俊红,等.香蕉根际促生菌的研究展望[J].热带作物学报,2011,32(1):182-187.
[16]刘佳莉,方芳,史煦涵,等.2株盐碱地燕麦根际促生菌的筛选及其促生作用研究[J].草业学报,2013,22(2):132-139.
[17]康贻军,程洁,梅丽娟,等.植物根际促生菌的筛选及鉴定.微生物学报,2010,50(7):853-861.
[18]Glick B R,Liu C,Ghosh S,et al.Early development of canola seedlings in the presence of the plant growth promoting rhizobacterium Pseudomonas putida GR 12-2[J].Soil Biology and Biochemistry,1997,29(8):1233-1239.
基本信息:
DOI:10.14096/j.cnki.cn34-1069/n.2015.02.017
中图分类号:S565.3;Q93
引用信息:
[1]陆娟,苏利梅,胡名扬,等.芝麻根际生长素产生菌SA4的分离与鉴定[J].阜阳师范学院学报(自然科学版),2015,32(02):79-82.DOI:10.14096/j.cnki.cn34-1069/n.2015.02.017.
基金信息:
国家自然科学基金(31201788);; 安徽省教育厅重点顶目(KJ2013A206);; 阜阳师范学院科技成果孵化基金(2013KJFH04);; 安徽省大学生创新创业训练计划项目(AH201310371046)资助
2015-06-16
2015-06-16