| 327 | 1 | 67 |
| 下载次数 | 被引频次 | 阅读次数 |
通过罗丹明6G与水杨醛缩合得到荧光探针YH，并采用~1H NMR和13C NMR表征了探针YH结构。该探针能够专一识别铜离子，通过紫外吸收光谱和荧光光谱研究YH对Cu2+的识别性质，并得到其最低检出限是1.347×10-6M。进一步采用红外光谱研究了两者间的作用机制。最后，通过试纸和线虫生物实验探究了YH的实际应用和生物成像的潜力。
Abstract：A fluorescence probe YH, which was synthesized by Rhodamine 6G and salicylaldehyde, made sure its structure by the following methods,~1H NMR,13C NMR. It could act with Cu2+ sensitively and specifically, and through ultraviolet and fluorescent spectrum, it could make sure identification nature between YH and Cu2+, the detection limit of this probe for Cu2+ was1.347× 10-6 M. The interaction mechanism between them was further studied by infrared experiment. Finally, a paper strip and nematodes experiment were carried out to study the practical application and application potential of biological imaging of YH.
[1] WANG Y, ZHU Z, FAN C B, et al. A naphthalene–dansylhydrazine based ratiometric fluorescence probe for selectively detecting Cu2+[J]. Tetrahedron Letters.2020, 61(04):151427-151430.
[2] JIA T, FU M, ZHANG M Y, et al. A novel cholesterol conjugated fluorescence probe for Cu2+detection and bioimaging in living cells[J]. Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy. 2020,227(09):117530.
[3] NI H Z, WANG Q M, JIN L, et al. High selectivity and reversibility/reusability red emitting fluorescent probe for copper ions detection and imaging in living cells[J]. Journal of Luminescence. 2019, 206:125-131.
[4] YI X Q, HE Y F, CAO Y S, et al. Porphyrinic probe for fluorescence"turn-on"monitoring of Cu2+in aqueous buffer and mitochondria[J]. ACS Sensor. 2019, 4(04):856-864.
[5] XU P P, LIU X N, LIU L, et al. Carbazole-based colorimetric and fluorescent probe for Cu2+and its utility in bio-imaging and real water samples[J]. Journal of the Chinese Chemical Society. 2020, 68(01):106-113.
[6] JIN M Z, JIN D D, WANG H W, et al. Ratiometric fluorescence imaging of Cu2+based on spirolactamized benzothiazole-substituted N,N-diethylrhodol probe[J].Journal of Molecular Structure. 2021, 1226:129360.
[7]杨亚成，赵永梅，王清照，等．一种裸眼识别的反应型铜离子荧光探针[J]．精细化工，2018,35(04):569-579.
[8] FANG R, DING X, LUO W, et al. An“off-on”near-infrared fluorescent probe for Cu2+detection in living cells[J]. Chinese Journal of Organic Chemistry. 2020,40(09):2949-2955.
[9]武小明，王皓，杨绍祥，等．一种检测铜离子的荧光探针及其应用[J]．精细化工，2020,37(09):1769-1774.
[10]贾斌，李漫，郑嘉，等．一种高效检测铜离子的罗丹明类荧光探针的构建及应用[J].化学试剂，2021,43(03):370-375.
[11] GARCIA-BELTRAN O, MENA N, FRIEDRICH L C,et al. Design and synthesis of a new coumarin-based‘turn-on’fluorescent probe selective for Cu2+[J]. Tetrahedron Letters. 2012, 53(39):5280-5283.
[12] MENG X, WANG L, ZHAI Y G, et al. Electron-donating methoxy group enhances the stability and efficiency of indole-based fluorescent probe for detecting Cu2+[J]. Research on Chemical Intermediates. 2020, 46(12):5517-5533.
[13] XU Z Y, HU L, YUAN J, et al. A fluorescence probe for metal ions based on black phosphorus quantum dots[J]. Advanced Materials Interfaces. 2020, 7(07):1902075.
[14]姜秀娟，朱荣贵，徐曼，等．铜(Ⅱ)荧光探针的合成及其识别性能研究[J]．分析试验室，2017,36(07):775-777.
[15] CHO S W, RAO A S, BHUNIA S, et al. Ratiometric fluorescence detection of Cu(II)with a keto-dipicolylamine ligand:A mechanistic implication[J]. Sensors and Actuators B:Chemical. 2019, 279:204-212.
[16] XIAO N, ZHANG C. Selective monitoring of Cu(II)with a fluorescence-on naphthalene–based probe in aqueous solution[J]. Inorganic Chemistry Communications. 2019, 107:107467.
[17]边永军，渠星宇，李芬芳，等．荧光猝灭型硼氟二吡咯类Cu2+探针的合成及其生物细胞成像的应用[J]．无机化学学报，2021,37(06):1089-1096.
[18]崔爱军，黄伟，何明阳，等．基于Schiff碱结构的Cu2+荧光探针的合成及性能研究[J]．化学试剂，2010,32(11):1019-1023.
[19] FU Y, PANG X X, WANG Z Q, et al. A highly sensitive and selective fluorescent probe for determination of Cu(II)and application in live cell imaging[J]. Spectrochim Acta A Mol Biomol Spectrosc. 2019, 208:198-205.
[20]许丙嵩，陈浩，胡磊，等．基于铱配合物的组氨酸荧光探针的设计合成[J]．阜阳师范大学学报(自然科学版)，2021,38(04):24-30.
[21]张杰，戴红亮，郑琦君，等．一种识别Cu2+的罗丹明衍生物的合成及其性质研究[J]．阜阳师范大学学报(自然科学版)，2022,39(01):47-54.
[22] TU H, CHEN H Y, WU C Y, et al. Responsive fluorescence enhancement for in vivo Cu(II)monitoring in zebrafish larvae[J]. Biosens Bioelectron. 2022, 200:113885-113893.
[23] LIU J L, MENG X, DUAN H D, et al. Two Schiffbase fluorescence probes based on triazole and benzotriazole for selective detection of Zn2+[J]. Sensors and Actuators B:Chemical. 2016. 227:296-303.
[24] WANG F W, DUAN H D, XING D X, et al. Novel turn-on fluorescence probes for Al3+based on conjugated pyrazole Schiff base[J]. Journal of Fluorescence.2017, 27(05):1721-1727.
[25]张杰，白翠冰，覃于鑫，等.一种双取代马来腈衍生物的合成[J]．阜阳师范大学学报(自然科学版)，2020,37(01):33-37+43.
[26] DING Y Z, GONG H L, WANG K P. Highly sensitive and selective turn-on fluorescent probe for Hg2+based on Rhodamine 6G-thiourea conjugate[J]. Journal of Fluorescence. 2017, 27(03):1095-1099.
[27]胡向阳，常玲，马静，等．含吡啶基席夫碱的合成和光学性质[J]．阜阳师范学院学报(自然科学版)，2016,33(02):36-40.
[28] WU W N, WU H, ZHONG R B, et al. Ratiometric fluorescent probe based on pyrrole-modified rhodamine6G hydrazone for the imaging of Cu2+in lysosomes[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy. 2019, 212:121-127.
基本信息:
DOI：10.14096/j.cnki.cn34-1069/n/2096-9341(2022)04-0013-07
中图分类号:O657.3
引用信息:
[1]覃于鑫,陈大朔,何书平,等.一种基于罗丹明6G的“关——开”型铜离子探针[J].阜阳师范大学学报(自然科学版),2022,39(04):13-19.DOI:10.14096/j.cnki.cn34-1069/n/2096-9341(2022)04-0013-07.
基金信息:
国家自然科学基金项目（21302019）; 安徽自然科学基金项目（1908085QB78）; 安徽省高校自然科学研究项目（KJ2021A0667,KJ2020A05250,KJ2021A0673）; 高校优秀拔尖人才培育资助项目（gxyqZD2022065）; 大学生创新创业项目（202110371004,S202110371019）资助
2022-12-15
2022-12-15