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为探讨重度抑郁症（MDD）合并睡眠障碍的发病机制，分析了GEO数据库的GSE98793数据集，获得MDD的差异表达基因（DEGs），并利用Genecards数据库筛选出睡眠障碍相关基因（GRSDs），对DEGs与GRSDs取交集得到睡眠障碍相关差异表达基因（SDRDEGs）。对SDRDEGs进行GO/KEGG分析，对SDRDEGs构建蛋白质-蛋白质相互作用（PPI）网络以鉴定核心基因。进一步分析核心基因的miRNA、转录因子相互作用，以及其诊断价值。结果显示，SDRDEGs显著富集于与神经炎症和生物钟调节相关的通路，表明它们在MDD的发病中发挥作用。核心基因EGF(AUC=0.621)、EPO(AUC=0.603)、MPO(AUC=0.615)、MAPK14(AUC=0.614)、KCNA3(AUC=0.624)、RORA(AUC=0.651)和NFATC2(AUC=0.611)有诊断价值，是MDD患者的有效生物标志物。本研究强调了睡眠障碍与抑郁症之间的复杂关系，深入研究与睡眠障碍相关的差异表达基因，可能会为改善抑郁症的治疗结果提供新的方法。
Abstract：To explore the pathogenesis of major depressive disorder(MDD) combined with sleep disorders, the GSE98793 dataset from the GEO database was analyzed to identify differentially expressed genes(DEGs) in MDD, and sleep disorder-related genes(GRSDs) were screened using the Genecards database. The intersection of DEGs and GRSDs was taken to obtain sleep disorder-related differentially expressed genes(SDRDEGs). GO/KEGG analysis was performed on SDRDEGs. A protein-protein interaction(PPI) network was constructed for SDRDEGs to identify hub genes. Furthermore, interactions between hub genes' miRNAs and transcription factors, and their diagnostic value were analyzed. The results showed that SDRDEGs were significantly enriched in pathways related to neuroinflammation and circadian rhythm regulation, indicating their role in the pathogenesis of MDD. Hub genes EGF(AUC=0.621), EPO(AUC=0.603), MPO(AUC=0.615), MAPK14(AUC=0.614), KCNA3(AUC=0.624), RORA(AUC=0.651), and NFATC2(AUC=0.611) have diagnostic value and are considered effective biomarkers for MDD patients. This study emphasizes the complex relationship between sleep disorders and depression, suggesting that in-depth research on differentially expressed genes related to sleep disorders may provide new approaches to improve treatment outcomes for depression.
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基本信息:
DOI：10.14096/j.cnki.cn34-1334/n.2025.12.006
中图分类号:R749.4;R740
引用信息:
[1]刘丽云,薛博文,潘婷,等.基于生物信息学数据的重度抑郁症的睡眠障碍机制研究[J].阜阳师范大学学报(自然科学版),2025,42(04):38-46.DOI:10.14096/j.cnki.cn34-1334/n.2025.12.006.
基金信息:
安徽省创新训练项目（S02310371100）; 阜阳师范大学自然科学研究项目（2021HXYXZX）;阜阳师范大学横向医学专项一般项目（2022HXYXZX08）;阜阳师范大学横向医学专项项目（2024FYNUFN19）
2025-12-15
2025-12-15